Rad52’s DNA annealing activity drives template switching associated with restarted DNA replication

Anastasiya Kishkevich; Sanjeeta Tamang; Michael O. Nguyen; Judith Oehler; Elena Bulmaga; Christos Andreadis; Carl A. Morrow; Manisha Jalan; Fekret Osman; Matthew C. Whitby

doi:10.1038/s41467-022-35060-4

Nature Communications (Nov 2022)

Rad52’s DNA annealing activity drives template switching associated with restarted DNA replication

Anastasiya Kishkevich,
Sanjeeta Tamang,
Michael O. Nguyen,
Judith Oehler,
Elena Bulmaga,
Christos Andreadis,
Carl A. Morrow,
Manisha Jalan,
Fekret Osman,
Matthew C. Whitby

Affiliations

Anastasiya Kishkevich: Department of Biochemistry, University of Oxford
Sanjeeta Tamang: Department of Biochemistry, University of Oxford
Michael O. Nguyen: Department of Biochemistry, University of Oxford
Judith Oehler: Department of Biochemistry, University of Oxford
Elena Bulmaga: Department of Biochemistry, University of Oxford
Christos Andreadis: Department of Biochemistry, University of Oxford
Carl A. Morrow: Department of Biochemistry, University of Oxford
Manisha Jalan: Department of Biochemistry, University of Oxford
Fekret Osman: Department of Biochemistry, University of Oxford
Matthew C. Whitby: Department of Biochemistry, University of Oxford

DOI: https://doi.org/10.1038/s41467-022-35060-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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The restart of collapsed replication forks is associated with high rates of genomic rearrangement. Here, the authors show that during restart initiation rearrangements are driven mainly by Rad51, whereas during elongation they rely more on Rad52.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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