Effects of resistance, endurance, and concurrent exercise on carnitine palmitoyltransferases 1, 2 and fatigue index in obese diabetic rats

Abstract

The objective of this study was to evaluate the effects of endurance, resistance, and Concurrent endurance and resistance training on fat oxidation and fatigue levels in obese diabetic rats. A total of forty overweight rats (average weight of 380 ± 50 g) were randomly assigned to five distinct groups. Diabetes was induced through peritoneal administration of Streptozotocin at a dosage of 55 mg/kg body weight. Endurance training was conducted via moderate-intensity treadmill running (50%-70% of maximum running capacity) for a duration of eight weeks, with sessions lasting one hour per day, five days a week. Resistance training involved ladder climbing at 50-70% of maximum load, also for eight weeks, with five sessions per week, comprising 15 climbs per session and a one-minute rest interval between climbs. The Concurrent training regimen alternated between treadmill and ladder exercises. Levels of CPT1 and CPT2 were quantified using rat CPT1 and CPT2 ELISA kits, both prior to and 48 hours following the training period. Fatigue indices were assessed as well. All three training modalities significantly influenced CPT1 levels (F=93.35, P=0.001) and led to a notable increase in CPT2 levels (F=26.76, P=0.001). Additionally, significant alterations in fatigue indices were observed (F=26.12, P=0.001). The findings suggest that aerobic, resistance, and Concurrent training regimens can enhance metabolic function and prolong the duration until fatigue in elderly diabetic rats. Nonetheless, the extent of these improvements is somewhat contingent upon the specific type of exercise performed, with aerobic and Concurrent training demonstrating a more pronounced effect compared to resistance training.

Keywords

• cpt1
• cpt2
• training
• diabetes
• body weight