Optimization of nutrient medium composition for BHK-21/2-17 suspension culture

Abstract

The paper demonstrates results of the research aimed at optimization of amino acid composition of the nutrient media used for BHK-21-2/17 suspension cultivation by change of amino acid amount in such media. Growth level in the control and tested media amounted to 5.6; cell growth dynamics was also similar. In-media utilization of different amino acids was different. The cells mostly consumed glutamin, serine, tyrosine, methionin (up to 90%, 55%, 40% and 60%, respectively). Growth of alanine level was reported (from 10% in the control sample and up to 60% in the test sample). Amount of tryptophan and glutamic acid remained stable. Glycine, threonine, isoleucine were actively utilized within the first 24 hours (7-13%, 10-18%, 34-41%, respectively in the control experiment). Hereafter, the concentration slightly increased (by 7-45%, by 16-21%, by 20-50%, respectively in the control experiment). During the FMDV reproduction in the cells grown in the test and control media no significant differences in the yield of immunogenic virus components were reported.

Keywords

• клетки внк-21/2-17
• аминокислоты
• аминокислотный состав
• гидролизат белков крови
• питательная среда
• bhk-21/2-17 cells
• amino acids
• amino acid composition
• blood-derived protein hydrolysate
• nutrient medium