Evaluation of post-thaw quality of brown-swiss and holstein bull semen diluted with different extenders

Abstract

Aim: To evaluate post-thaw guality of Brown-Swiss and Holstein bull semen diluted with different extenders. Materials and Methods: Ejaculates obtained from Holstein (n=36) and Brown-Swiss (n=36) bulls were divided into three aliquots and diluted with Tris-based, Optidyl® and Bioxcell® extenders, respectively. Thereafter they were frozen and thawed following a standard protocol. The effectiveness of freezing extenders was assessed according to postthaw sperm motility, acrosomal and total abnormalities and plasma membrane integrity. Results: With respect to Holstein bull semen, the highest percentages of sperm subjective (p®. Optidyl® extender also provided best protection in terms of acrosome and plasma membrane integrity compared to other extenders (p®. The percentage of progressive motility was found to be higher in Optidyl® than Bioxcell® (p® extender compared to the other groups (p Conclusion: Optidyl® extender may be preferred rather than Bioxcell® or Tris + egg yolk extenders for freezing the Holstein and Brown-Swiss bull semen.

Keywords

• bull semen
• cryopreservation
• extender
• membrane integrity
• seminal parameters