MicroRNA Signatures and Molecular Subtypes of Glioblastoma: The Role of Extracellular Transfer

Jakub Godlewski; Ruben Ferrer-Luna; Arun K. Rooj; Marco Mineo; Franz Ricklefs; Yuji S. Takeda; M. Oskar Nowicki; Elżbieta Salińska; Ichiro Nakano; Hakho Lee; Ralph Weissleder; Rameen Beroukhim; E. Antonio Chiocca; Agnieszka Bronisz

doi:10.1016/j.stemcr.2017.04.024

Stem Cell Reports (Jun 2017)

MicroRNA Signatures and Molecular Subtypes of Glioblastoma: The Role of Extracellular Transfer

Jakub Godlewski,
Ruben Ferrer-Luna,
Arun K. Rooj,
Marco Mineo,
Franz Ricklefs,
Yuji S. Takeda,
M. Oskar Nowicki,
Elżbieta Salińska,
Ichiro Nakano,
Hakho Lee,
Ralph Weissleder,
Rameen Beroukhim,
E. Antonio Chiocca,
Agnieszka Bronisz

Affiliations

Jakub Godlewski: Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Ruben Ferrer-Luna: Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Cancer Program, BROAD Institute of MIT and Harvard, Cambridge, MA 02142, USA
Arun K. Rooj: Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Marco Mineo: Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Franz Ricklefs: Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Yuji S. Takeda: Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
M. Oskar Nowicki: Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Elżbieta Salińska: Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences 02-106 Warsaw, Poland
Ichiro Nakano: Department of Neurosurgery and Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35243, USA
Hakho Lee: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Ralph Weissleder: Department of Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Rameen Beroukhim: Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Cancer Program, BROAD Institute of MIT and Harvard, Cambridge, MA 02142, USA
E. Antonio Chiocca: Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Agnieszka Bronisz: Department of Neurosurgery, Harvey Cushing Neuro-oncology Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA

DOI: https://doi.org/10.1016/j.stemcr.2017.04.024
Journal volume & issue: Vol. 8, no. 6
pp. 1497 – 1505

Abstract

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Despite the importance of molecular subtype classification of glioblastoma (GBM), the extent of extracellular vesicle (EV)-driven molecular and phenotypic reprogramming remains poorly understood. To reveal complex subpopulation dynamics within the heterogeneous intratumoral ecosystem, we characterized microRNA expression and secretion in phenotypically diverse subpopulations of patient-derived GBM stem-like cells (GSCs). As EVs and microRNAs convey information that rearranges the molecular landscape in a cell type-specific manner, we argue that intratumoral exchange of microRNA augments the heterogeneity of GSC that is reflected in highly heterogeneous profile of microRNA expression in GBM subtypes.

Published in Stem Cell Reports

ISSN: 2213-6711 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: http://www.cell.com/stem-cell-reports/home

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