Emerging Infectious Diseases (Jul 2018)

Use of Urea Wash ELISA to Distinguish Zika and Dengue Virus Infections

  • Wen-Yang Tsai,
  • Han Ha Youn,
  • Jasmine Tyson,
  • Carlos Brites,
  • Jih-Jin Tsai,
  • Celia Pedroso,
  • Jan Felix Drexler,
  • Angel Balmaseda,
  • Eva Harris,
  • Wei-Kung Wang

DOI
https://doi.org/10.3201/eid2407.171170
Journal volume & issue
Vol. 24, no. 7
pp. 1355 – 1359

Abstract

Read online

Serologic testing remains crucial for Zika virus diagnosis. We found that urea wash in a Zika virus nonstructural protein 1 IgG ELISA distinguishes secondary dengue virus infection from Zika virus infection with previous dengue (sensitivity 87.5%, specificity 93.8%). This test will aid serodiagnosis, serosurveillance, and monitoring of Zika complications in dengue-endemic regions.

Keywords