PLoS ONE (Jan 2021)

BC-store: A program for MGISEQ barcode sets analysis.

  • Irina Bulusheva,
  • Vera Belova,
  • Boris Nikashin,
  • Dmitriy Korostin

DOI
https://doi.org/10.1371/journal.pone.0247532
Journal volume & issue
Vol. 16, no. 3
p. e0247532

Abstract

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Here we present the devised BC-store-a program for analyzing and selecting sets of barcodes for sequencing on platforms manufactured by MGI Tech (China). The app is available as an open source in Python3 and as a desktop version. The application allows analyzing the compatibility of barcodes on a single lane of a flow cell in a set in the case of equal and arbitrary fractions. In addition, with the help of this tool barcodes can be added to an existing set with custom share options. In this paper we describe how BC-store works for different tasks and consider the effectiveness of using BC-store in sequence lab routine tasks.