COMPARATIVE MOLECULAR GENETIC ANALYSIS BETWEEN UKRAINIAN AND EU REGISTERED GLYPHOSATE-TOLERANT RAPESEED TRANSGENIC PLANTS

Abstract

The purpose of research was to analyze 10 developed at the Institute of Cell Biology and Genetic Engineering lines of rapeseed to confirm the presence and functionality of the transferred transgene CP4 epsps, as well as the differences among those lines from registered transformation events GT73 and GT200 (Monsanto). During the study extraction of total rapeseed DNA, PCR analysis, electrophoretic separation and visualization of amplicons in agarose gel were conducted, as well as testing of green plants for resistance to glyphosate in greenhouse. The structural difference among 7 transgenic lines from registered transformation events GT73 and GT200 was revealed. Plants showing the presence of synthetic CP4 epsps sequence were resistant to the herbicide in a closed soil. The uniqueness of the obtained transformation events was confirmed, as well as the prospect of using them in breeding.

Keywords

• rapeseed
• glyphosate
• genetic transformation
• CP4 epsps gene