Human Genomics (Jun 2023)

Modeling the longitudinal changes of ancestry diversity in the Million Veteran Program

  • Frank R. Wendt,
  • Gita A. Pathak,
  • Jacqueline Vahey,
  • Xuejun Qin,
  • Dora Koller,
  • Brenda Cabrera-Mendoza,
  • Angela Haeny,
  • Kelly M. Harrington,
  • Nallakkandi Rajeevan,
  • Linh M. Duong,
  • Daniel F. Levey,
  • Flavio De Angelis,
  • Antonella De Lillo,
  • Tim B. Bigdeli,
  • Saiju Pyarajan,
  • VA Million Veteran Program,
  • John Michael Gaziano,
  • Joel Gelernter,
  • Mihaela Aslan,
  • Dawn Provenzale,
  • Drew A. Helmer,
  • Elizabeth R. Hauser,
  • Renato Polimanti,
  • Department of Veteran Affairs Cooperative Study Program (#2006)

DOI
https://doi.org/10.1186/s40246-023-00487-3
Journal volume & issue
Vol. 17, no. 1
pp. 1 – 11

Abstract

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Abstract Background The Million Veteran Program (MVP) participants represent 100 years of US history, including significant social and demographic changes over time. Our study assessed two aspects of the MVP: (i) longitudinal changes in population diversity and (ii) how these changes can be accounted for in genome-wide association studies (GWAS). To investigate these aspects, we divided MVP participants into five birth cohorts (N-range = 123,888 [born from 1943 to 1947] to 136,699 [born from 1948 to 1953]). Results Ancestry groups were defined by (i) HARE (harmonized ancestry and race/ethnicity) and (ii) a random-forest clustering approach using the 1000 Genomes Project and the Human Genome Diversity Project (1kGP + HGDP) reference panels (77 world populations representing six continental groups). In these groups, we performed GWASs of height, a trait potentially affected by population stratification. Birth cohorts demonstrate important trends in ancestry diversity over time. More recent HARE-assigned Europeans, Africans, and Hispanics had lower European ancestry proportions than older birth cohorts (0.010 < Cohen’s d < 0.259, p < 7.80 × 10−4). Conversely, HARE-assigned East Asians showed an increase in European ancestry proportion over time. In GWAS of height using HARE assignments, genomic inflation due to population stratification was prevalent across all birth cohorts (linkage disequilibrium score regression intercept = 1.08 ± 0.042). The 1kGP + HGDP-based ancestry assignment significantly reduced the population stratification (mean intercept reduction = 0.045 ± 0.007, p < 0.05) confounding in the GWAS statistics. Conclusions This study provides a characterization of ancestry diversity of the MVP cohort over time and compares two strategies to infer genetically defined ancestry groups by assessing differences in controlling population stratification in genome-wide association studies.

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