miR-26a-5p Regulates Adipocyte Differentiation via Directly Targeting ACSL3 in Adipocytes
Ning Ding,
Wenwen Wang,
Jun Teng,
Yongqing Zeng,
Qin Zhang,
Licai Dong,
Hui Tang
Affiliations
Ning Ding
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science & Technology, Shandong Agricultural University, Taian, Shandong Province, China
Wenwen Wang
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science & Technology, Shandong Agricultural University, Taian, Shandong Province, China
Jun Teng
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science & Technology, Shandong Agricultural University, Taian, Shandong Province, China
Yongqing Zeng
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science & Technology, Shandong Agricultural University, Taian, Shandong Province, China
Qin Zhang
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science & Technology, Shandong Agricultural University, Taian, Shandong Province, China
Licai Dong
Shandong Futong Agriculture & Animal Husbandry Development Co. LTD, Linyi, China
Hui Tang
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science & Technology, Shandong Agricultural University, Taian, Shandong Province, China
ABSTRACTPreadipocytes become mature adipocytes after proliferation and differentiation, and although many genes and microRNAs have been identified in intramuscular fat, their physiological function and regulatory mechanisms remain largely unexplored. miR-26a-5p has been reported to be related to fat deposition, but its effect on porcine preadipocyte differentiation has not been explored. In this study, bioinformatics analysis and luciferase reporter assay identified that miR-26a-5p binds to the 3ʹUTR of Acyl-CoA synthetase long-chain family member 3 (ACSL3) mRNA. The model for porcine intramuscular preadipocyte differentiation was established to explore the function of miR-6a-5p-ACSL3 on adipocyte differentiation. ACSL3 knockdown markedly reduced the triglycerides (TG) content of cells, as well as the mRNA levels of adipogenic marker genes (PPAR-γ and SREBP-1c). The number of lipid droplets in cells transfected with a miR-26a-5p mimic is significantly reduced, consistent with ACSL3 knockdown results, while the miR-26a-5p inhibitor resulted in opposite results. Taken together, miR-26a-5p is a repressor of porcine preadipocyte differentiation and plays a vital role in ACSL3-mediated adipogenesis.
