Queratina a partir de la hidrólisis enzimática de harina de plumas de pollo, utilizando queratinasas producidas por Bacillus subtilis

Abstract

The objective of this research was hydrolyzed feather meal of chicken for the production of keratin. The methodological research design was experimental; four fermentations, corresponding to a completely randomized experimental design, resulting from considering factors [substrate concentration (18 g / L and 23 g / L) and concentration of inoculum (2 and 3 g / L), two levels was performed for each factor]. Determining the total nitrogen concentration was performed by UV-Visible spectrophotometry, the method used is the persulfate potassium digestion (K2S2O8), the maximum concentration of nitrogen is reached in the treatment 2 (1.5%) which multiplied by the factor protein 6.25 gives a percentage of 9.4% protein in the hydrolyzed keratin, showing significant difference (p<0.05) from other treatments at 24 hours hydraulic retention. In conclusion the enzymatic hydrolysis of chicken feather meal is possible using the Bacillus subtilis as a producer of proteases.

Keywords

• fermentation
• UV-visible spectrophotometry
• digestion
• enzymatic hydrolysis
• proteases