PLoS ONE (Jan 2013)

Relationship of C5L2 receptor to skeletal muscle substrate utilization.

  • Christian Roy,
  • Sabina Paglialunga,
  • Gert Schaart,
  • Esther Moonen-Kornips,
  • Ruth C Meex,
  • Esther Phielix,
  • Joris Hoeks,
  • Matthijs K C Hesselink,
  • Katherine Cianflone,
  • Patrick Schrauwen

DOI
https://doi.org/10.1371/journal.pone.0057494
Journal volume & issue
Vol. 8, no. 2
p. e57494

Abstract

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ObjectiveTo investigate the role of Acylation Stimulating Protein (ASP) receptor C5L2 in skeletal muscle fatty acid accumulation and metabolism as well as insulin sensitivity in both mice and human models of diet-induced insulin resistance.Design and methodsMale wildtype (WT) and C5L2 knockout (KO) mice were fed a low (LFD) or a high (HFD) fat diet for 10 weeks. Intramyocellular lipid (IMCL) accumulation (by oil red O staining) and beta-oxidation HADH enzyme activity were determined in skeletal muscle. Mitochondria were isolated from hindleg muscles for high-resolution respirometry. Muscle C5L2 protein content was also determined in obese type 2 diabetics and age- and BMI matched men.ResultsIMCL levels were increased by six-fold in C5L2KO-HFD compared to WT-HFD mice (pConclusionThe results suggest that insulin sensitivity may be permissive for coupling of C5L2 levels to lipid storage and utilization.