Journal of Immunology Research (Jan 2015)

Decreased Frequency of Circulating Myelin Oligodendrocyte Glycoprotein B Lymphocytes in Patients with Relapsing-Remitting Multiple Sclerosis

  • Annie Elong Ngono,
  • Maud Lepetit,
  • Markus Reindl,
  • Alexandra Garcia,
  • Flora Guillot,
  • Athénaïs Genty,
  • Mélanie Chesneau,
  • Marion Salou,
  • Laure Michel,
  • Fabienne Lefrere,
  • Kathrin Schanda,
  • Berthe-Marie Imbert-Marcille,
  • Nicolas Degauque,
  • Arnaud Nicot,
  • Sophie Brouard,
  • David-Axel Laplaud,
  • Jean-Paul Soulillou

DOI
https://doi.org/10.1155/2015/673503
Journal volume & issue
Vol. 2015

Abstract

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Although there is no evidence for a role of anti-MOG antibodies in adult MS, no information on B lymphocytes with MOG-committed BCR is available. We report here on the frequency of anti-MOG B cells forming rosettes with polystyrene beads (BBR) covalently bound to the extracellular domain of rhMOG in 38 relapsing-remitting patients (RRMS) and 50 healthy individuals (HI). We show a substantial proportion of circulating anti-MOG-BBR in both RRMS and HI. Strikingly, MOG-specific B cells frequencies were lower in MS than in HI. Anti-MOG antibodies measured by a cell-based assay were not different between MS patients and controls, suggesting a specific alteration of anti-MOG B cells in MS. Although anti-MOG-BBR were higher in CNS fluid than in blood, no difference was observed between MS and controls. Lower frequency of MOG-BBR in MS was not explained by an increased apoptosis, but a trend for lower proliferative capacity was noted. Despite an efficient B cell transmigration across brain derived endothelial cells, total and anti-MOG B cells transmigration was similar between MS and HI. The striking alteration in MOG-specific B cells, independent of anti-MOG antibody titers, challenges our view on the role of MOG-specific B cells in MS.