Red Meat Intake, Indole-3-Acetate, and <i>Dorea longicatena</i> Together Affect Insulin Resistance after Gastric Bypass
Ana Paula Aguiar Prudêncio,
Danielle Cristina Fonseca,
Natasha Mendonça Machado,
Juliana Tepedino Martins Alves,
Priscila Sala,
Gabriel R. Fernandes,
Raquel Susana Torrinhas,
Dan Linetzky Waitzberg
Affiliations
Ana Paula Aguiar Prudêncio
Laboratory of Nutrition and Metabolic Surgery of the Digestive System, LIM 35, Department of Gastroenterology, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, SP, Brazil
Danielle Cristina Fonseca
Laboratory of Nutrition and Metabolic Surgery of the Digestive System, LIM 35, Department of Gastroenterology, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, SP, Brazil
Natasha Mendonça Machado
Laboratory of Nutrition and Metabolic Surgery of the Digestive System, LIM 35, Department of Gastroenterology, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, SP, Brazil
Juliana Tepedino Martins Alves
Hospital Sírio Libanês, Department of Medical Clinical Nutrition, Brasilia 70200-730, DF, Brazil
Priscila Sala
Faculty of Nutrition, Universidade São Camilo, São Paulo 04263-200, SP, Brazil
Gabriel R. Fernandes
Instituto Rene Rachou, Fiocruz Minas, Belo Horizonte 30190-002, MG, Brazil
Raquel Susana Torrinhas
Laboratory of Nutrition and Metabolic Surgery of the Digestive System, LIM 35, Department of Gastroenterology, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, SP, Brazil
Dan Linetzky Waitzberg
Laboratory of Nutrition and Metabolic Surgery of the Digestive System, LIM 35, Department of Gastroenterology, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01246-903, SP, Brazil
Roux-en-Y Gastric bypass (RYGB) promotes improvement in type 2 diabetes (T2D) shortly after surgery, with metabolic mechanisms yet to be elucidated. This study aimed to investigate the relationship between food intake, tryptophan metabolism, and gut microbiota on the glycemic control of obese T2D women after RYGB surgery. Twenty T2D women who underwent RYGB were evaluated before and three months after surgery. Food intake data were obtained by a seven-day food record and a food frequency questionnaire. Tryptophan metabolites were determined by untargeted metabolomic analysis, and the gut microbiota was determined by 16S rRNA sequencing. The glycemic outcomes were fasting blood glucose, HbA1C, HOMA-IR, and HOMA-beta. Linear regression models were applied to assess the associations between the changes in food intake, tryptophan metabolism, and gut microbiota on glycemic control after RYGB. All variables changed after RYGB (p Dorea longicatena was associated with postoperative HOMA-IR {R2 0.80, R2 adj 0.74; p Dorea longicatena increased in the same period. These combined variables were associated with better insulin resistance in T2D women after RYGB.