Interaction of <i>Thalassia testudinum</i> Metabolites with Cytochrome P450 Enzymes and Its Effects on Benzo(a)pyrene-Induced Mutagenicity
Livan Delgado-Roche,
Rebeca Santes-Palacios,
José A. Herrera,
Sandra L. Hernández,
Mario Riera,
Miguel D. Fernández,
Fernando Mesta,
Gabino Garrido,
Idania Rodeiro,
Jesús Javier Espinosa-Aguirre
Affiliations
Livan Delgado-Roche
Departamento de Farmacología, Instituto de Ciencias del Mar (ICIMAR), Loma 14, Alturas del Vedado, Plaza de la Revolución, La Habana 10600, Cuba
Rebeca Santes-Palacios
Laboratorio de Toxicología Genética, Instituto Nacional de Pediatría, Insurgentes Sur 3700, Insurgentes Cuicuilco, Ciudad de México 04530, Mexico
José A. Herrera
Instituto de Ciencia y Tecnología de Materiales (IMRE), Universidad de La Habana, Zapata y G, Vedado, Plaza de la Revolución, La Habana 10400, Cuba
Sandra L. Hernández
Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Ciudad de México 04510, Mexico
Mario Riera
Departamento de Farmacología, Instituto de Ciencias del Mar (ICIMAR), Loma 14, Alturas del Vedado, Plaza de la Revolución, La Habana 10600, Cuba
Miguel D. Fernández
Departamento de Farmacología, Instituto de Ciencias del Mar (ICIMAR), Loma 14, Alturas del Vedado, Plaza de la Revolución, La Habana 10600, Cuba
Fernando Mesta
Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 239, Ciudad de México 07320, Mexico
Gabino Garrido
Departamento de Ciencias Farmacéuticas, Facultad de Ciencias, Universidad Católica del Norte, Angamos 0610, Antofagasta 1240000, Chile
Idania Rodeiro
Departamento de Farmacología, Instituto de Ciencias del Mar (ICIMAR), Loma 14, Alturas del Vedado, Plaza de la Revolución, La Habana 10600, Cuba
Jesús Javier Espinosa-Aguirre
Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Ciudad de México 04510, Mexico
The aim of the present work was to evaluate the effects of Thalassia testudinum hydroethanolic extract, its polyphenolic fraction and thalassiolin B on the activity of phase I metabolizing enzymes as well as their antimutagenic effects. Spectrofluorometric techniques were used to evaluate the effect of tested products on rat and human CYP1A and CYP2B activity. The antimutagenic effect of tested products was evaluated in benzo[a]pyrene (BP)-induced mutagenicity assay by an Ames test. Finally, the antimutagenic effect of Thalassia testudinum (100 mg/kg) was assessed in BP-induced mutagenesis in mice. The tested products significantly (p p Thalassia testudinum (100 mg/kg) significantly reduced (p Thalassia testudinum metabolites exhibit antigenotoxic activity mediated, at least, by the inhibition of CYP1A1-mediated BP biotransformation, arresting the oxidative and mutagenic damage. Thus, the metabolites of T. testudinum may represent a potential source of chemopreventive compounds for the adjuvant therapy of cancer.
