The complete mitochondrial genome of Semblis atrata (Trichoptera: Phryganeidae)

Abstract

Semblis atrata is one of three Semblis species distributed in clean brooks and streams in northern Eurasia. Genomic DNA of an S. atrata sample was extracted and sequenced for assembly and annotation of its complete mitogenome. The complete mitochondrial genome of S. atrata was 14,909 bp in length and consisted of 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes. The S. atrata COX1 gene features a CGA start codon, and COX1, COX2, ND1, and ND5 exhibit incomplete stop codons that are presumed to be completed by the addition of 3’ A residues to the mRNA. The nucleotide composition was highly AT biased, accounting for 77.71% of the whole mitogenome. Phylogenetic analysis placed Semblis as sister to Eubasilissa. The complete mitochondrial genome will be helpful for further studies on the population genetics of this species and phylogenetic analyses of Trichoptera.

Keywords

• illumina sequencing
• mitogenomics
• phylogeny
• semblis atrata