Electrochemical Aptasensor Based on Au Nanoparticles Decorated Porous Carbon Derived from Metal-Organic Frameworks for Ultrasensitive Detection of Chloramphenicol
Jing Yang,
Jiamin Zou,
Wei Zhong,
Jin Zou,
Yansha Gao,
Shuwu Liu,
Songbai Zhang,
Limin Lu
Affiliations
Jing Yang
Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, China
Jiamin Zou
Key Laboratory of Chemical Utilization of Plant Resources of Nanchang, College of Science, Jiangxi Agricultural University, Nanchang 330045, China
Wei Zhong
Key Laboratory of Chemical Utilization of Plant Resources of Nanchang, College of Science, Jiangxi Agricultural University, Nanchang 330045, China
Jin Zou
Key Laboratory of Chemical Utilization of Plant Resources of Nanchang, College of Science, Jiangxi Agricultural University, Nanchang 330045, China
Yansha Gao
Key Laboratory of Chemical Utilization of Plant Resources of Nanchang, College of Science, Jiangxi Agricultural University, Nanchang 330045, China
Shuwu Liu
Key Laboratory of Chemical Utilization of Plant Resources of Nanchang, College of Science, Jiangxi Agricultural University, Nanchang 330045, China
Songbai Zhang
Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, China
Limin Lu
Key Laboratory of Chemical Utilization of Plant Resources of Nanchang, College of Science, Jiangxi Agricultural University, Nanchang 330045, China
A facile and sensitive electrochemical aptamer sensor (aptasensor) based on Au nanoparticles-decorated porous carbon (AuNPs/PC) composite was developed for the efficient determination of the antibiotic drug chloramphenicol (CAP). AuNPs modified metal-organic framework (AuNPs/ZIF-8) is applied as a precursor to synthesize the porous carbon with homogeneous AuNPs distribution through a direct carbonization step under nitrogen atmosphere. The as-synthesized AuNPs/PC exhibits high surface area and improved conductivity. Moreover, the loading AuNPs could enhance the attachment of the aptamers on the surface of electrode through the Au–S bond. When added to CAP, poorly conductive aptamer-CAP complexes are formed on the sensor surface, which increases the hindrance to electron transfer resulting in a decrease in electrochemical signal. Based on this mechanism, the developed CAP aptasensor represents a wide linear detection range of 0.1 pM to 100 nM with a low detection limit of 0.03 pM (S/N = 3). In addition, the proposed aptasensor was employed for the analysis of CAP in honey samples and provided satisfactory recovery.
