Facile fabrication and biological investigations of metal oxides intercalated in kaolinite clay-based dressing material to improve wound healing ability in nursing care after post-operative period
Xia Cheng,
Jingjing Yao,
Wenhao Fan,
Li Zhen
Affiliations
Xia Cheng
Department of General Surgery, Nanfang Hospital, The First School of Clinical Medicine, Southern Medical University, Guangdong Provincial Engineering Technology Research Center of Minimally Invasive Surgery, Guangzhou, Guangdong Province, China; Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Guangzhou, Guangdong Province, China; Corresponding author. Department of General Surgery, Nanfang Hospital, The First School of Clinical Medicine, Southern Medical University, No.1838 North Guangzhou Avenue, Guangzhou 510515, Guangdong Province, China.
Jingjing Yao
Department of Hematology, Nanfang Hospital, The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
Wenhao Fan
Department of General Surgery, Nanfang Hospital, The First School of Clinical Medicine, Southern Medical University, Guangdong Provincial Engineering Technology Research Center of Minimally Invasive Surgery, Guangzhou, Guangdong Province, China; Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Guangzhou, Guangdong Province, China
Li Zhen
Department of General Surgery, Nanfang Hospital, The First School of Clinical Medicine, Southern Medical University, Guangdong Provincial Engineering Technology Research Center of Minimally Invasive Surgery, Guangzhou, Guangdong Province, China; Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Guangzhou, Guangdong Province, China; Corresponding author. Department of General Surgery, Nanfang Hospital, The First School of Clinical Medicine, Southern Medical University, No.1838 North Guangzhou Avenue, Guangzhou 510515, Guangdong Province, China.
The present investigation aims to design and development of hybrid zinc oxide (ZnO) and manganese dioxide (MnO2) nanoparticles (NPs) doped-biopolymer matrix-based cost-effective technique for the synthesis of biocompatible Kaolinite/Chitosan (Ka/CS) nanocomposites (NCs) could be used as agents for wound healing due to their efficiency and low toxicity. The crystallite size, phase purity and surface morphology of the synthesised NCs were investigated systemic analytical methods. The results revealed that the metal oxide nanocomposites presented that in rod-crystalline in shape and additionally exhibits that 20–30 nm in size. In vitro antibacterial analyses demonstrates that NCs have significantly improved bactericidal inhibition efficiency when compared to the bare hybrid NPs and polymeric components. The in vitro biocompatibility observation demonstrates that prepared hybrid-NPs encapsulated NCs have enhanced cell survival rate (>90 %), which was established by MTT assay and Live/Dead fluorescence assay methods at different incubation time. The DPPH assay was used to investigate the synergistic effects of prepared dressing materials increased antioxidant activity. Preliminary research indicates that these nanocomposites, ZnO/MnO2 incorporated and decorated with Ka/CS NCs, could be a significant promoter and potential candidate for use as a robust wound healing agent in post-operative nursing care.
