Inhibit Effect of Allium hirtifolium Boiss. (Persian shallot) Hydroalcoholic Extract on the Growth of Leishmania infantum in vitro

Abstract

Background: Antiprotozoal effects of genus Allium plants including garlic and onion have been proven in Aryana Lands since hundreds years ago. Objective: In this study, inhibitory effect of persian shallot hydroalcoholic extract on growth of Leishmania infantum was evaluated in vitro. Methods: Different concentrations of the extract including 0.01, 0.05, 0.1 and 0.2 mg/cc in schneider medium were prepared, the parasites were added and counted every day during 7 days after culture. Results: Concentrations between 0.01 - 0.1 mg/cc inhibited the growth of parasites on the third day, but the highest concentration (0.2 mg/cc) inhibited the growth of parasites on the first day. Conclusion: This study proves inhibitory effect of persian shallot hydroalcoholic extract (containing allicin, ajoene and other agents) on Leishmania infantum. For exact evaluation of persian shallot antileishmanian properties, it is necessary to evaluate inhibitory effect of the plant hydroalcoholic extract in vivo.

Keywords

• leishmania infantum
• persian shallot
• allium hirtifolium