Miniaturizable Chemiluminescence System for ATP Detection in Water
Giuseppe E. Capuano,
Domenico Corso,
Roberta Farina,
Gianni Pezzotti Escobar,
Giuseppe A. Screpis,
Maria Anna Coniglio,
Sebania Libertino
Affiliations
Giuseppe E. Capuano
Istituto per la Microeletttronica e Microsistemi—Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy
Domenico Corso
Istituto per la Microeletttronica e Microsistemi—Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy
Roberta Farina
Istituto per la Microeletttronica e Microsistemi—Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy
Gianni Pezzotti Escobar
URT “LabSens of Beyond Nano” of the Department of Physical Sciences and Technologies of Matter, National Research Council (CNR-DSFTM-ME), Viale Ferdinando Stagno D’Alcontres 31, 98166 Messina, Italy
Giuseppe A. Screpis
Department of Medical, Surgical Sciences and Advanced Technologies, University of Catania, Via S. Sofia 78, 95123 Catania, Italy
Maria Anna Coniglio
Istituto per la Microeletttronica e Microsistemi—Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy
Sebania Libertino
Istituto per la Microeletttronica e Microsistemi—Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy
We present the design, fabrication, and testing of a low-cost, miniaturized detection system that utilizes chemiluminescence to measure the presence of adenosine triphosphate (ATP), the energy unit in biological systems, in water samples. The ATP–luciferin chemiluminescent solution was faced to a silicon photomultiplier (SiPM) for highly sensitive real-time detection. This system can detect ATP concentrations as low as 0.2 nM, with a sensitivity of 79.5 A/M. Additionally, it offers rapid response times and can measure the characteristic time required for reactant diffusion and mixing within the reaction volume, determined to be 0.3 ± 0.1 s. This corresponds to a diffusion velocity of approximately 44 ± 14 mm2/s.
