Generation of human control iPS cell line CHOPWT10 from healthy adult peripheral blood mononuclear cells

Jean Ann Maguire; Alyssa L. Gagne; Chintan D. Jobaliya; Shilpa Gandre-Babbe; Paul Gadue; Deborah L. French

doi:10.1016/j.scr.2016.02.017

Stem Cell Research (Mar 2016)

Generation of human control iPS cell line CHOPWT10 from healthy adult peripheral blood mononuclear cells

Jean Ann Maguire,
Alyssa L. Gagne,
Chintan D. Jobaliya,
Shilpa Gandre-Babbe,
Paul Gadue,
Deborah L. French

Affiliations

Jean Ann Maguire: Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States
Alyssa L. Gagne: Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States
Chintan D. Jobaliya: Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States
Shilpa Gandre-Babbe: Coriell Institute for Medical Research, United States
Paul Gadue: Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States
Deborah L. French: Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States

DOI: https://doi.org/10.1016/j.scr.2016.02.017
Journal volume & issue: Vol. 16, no. 2
pp. 338 – 341

Abstract

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The CHOPWT10 iPS cell line was generated to be used as a control for applications such as in differentiation analyses to the three germ layers and derivative tissues. Peripheral blood mononuclear cells (PBMCs) obtained from a healthy adult male were reprogrammed using the non-integrating Sendai virus expressing Oct3/4, Sox2, c-Myc, and Klf4.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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