Synthesis, In Vitro Evaluation and Molecular Docking of the 5-Acetyl-2-aryl-6-hydroxybenzo[<i>b</i>]furans against Multiple Targets Linked to Type 2 Diabetes

Abstract

The 5-acetyl-2-aryl-6-hydroxybenzo[b]furans 2a−h have been evaluated through in vitro enzymatic assay against targets which are linked to type 2 diabetes (T2D), namely, α-glucosidase, protein tyrosine phosphatase 1B (PTP1B) and β-secretase. These compounds have also been evaluated for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging method. The most active compounds against α-glucosidase and/or PTP1B, namely, 4-fluorophenyl 2c, 4-methoxyphenyl 2g and 3,5-dimethoxyphenyl substituted 2h derivatives were also evaluated for potential anti-inflammatory properties against cyclooxygenase-2 activity. The Lineweaver-Burk and Dixon plots were used to determine the type of inhibition on compounds 2c and 2h against α-glucosidase and PTP1B receptors. The interactions were investigated in modelled complexes against α-glucosidase and PTP1B via molecular docking.

Keywords

• 5-acetyl-2-aryl-6-hydroxybenzo[<i>b</i>]furans
• α-glucosidase
• protein tyrosine phosphatase 1b
• β-secretase
• antioxidant activity
• cyclooxygenase-2
• molecular docking