Infrared spectroscopy is suitable for objective assessment of articular cartilage health

V. Virtanen; V. Tafintseva; R. Shaikh; E. Nippolainen; J. Haas; I.O. Afara; J. Töyräs; H. Kröger; J. Solheim; B. Zimmermann; A. Kohler; B. Mizaikoff; M. Finnilä; L. Rieppo; S. Saarakkala

Osteoarthritis and Cartilage Open (Jun 2022)

Infrared spectroscopy is suitable for objective assessment of articular cartilage health

V. Virtanen,
V. Tafintseva,
R. Shaikh,
E. Nippolainen,
J. Haas,
I.O. Afara,
J. Töyräs,
H. Kröger,
J. Solheim,
B. Zimmermann,
A. Kohler,
B. Mizaikoff,
M. Finnilä,
L. Rieppo,
S. Saarakkala

Affiliations

V. Virtanen: Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland; Corresponding author. Research Unit of Medical Imaging, Physics and Technology University of Oulu Aapistie 5 A, 90220, Oulu, Finland.
V. Tafintseva: Faculty of Science and Technology, Norwegian University of Life Sciences, Ås, Norway
R. Shaikh: Department of Applied Physics, University of Eastern Finland, Kuopio Campus, Kuopio, Finland
E. Nippolainen: Department of Applied Physics, University of Eastern Finland, Kuopio Campus, Kuopio, Finland
J. Haas: Institute of Analytical and Bioanalytical Chemistry, Ulm University, Ulm, Germany; Hahn-Schickard, Ulm, Germany
I.O. Afara: Department of Applied Physics, University of Eastern Finland, Kuopio Campus, Kuopio, Finland; School of Information Technology and Electrical Engineering, The University of Queensland, Brisbane, Australia
J. Töyräs: Department of Applied Physics, University of Eastern Finland, Kuopio Campus, Kuopio, Finland; Kuopio University Hospital, Kuopio, Finland
H. Kröger: Department of Orthopedics, Traumatology and Hand Surgery, Kuopio University Hospital, Kuopio, Finland
J. Solheim: Faculty of Science and Technology, Norwegian University of Life Sciences, Ås, Norway
B. Zimmermann: Faculty of Science and Technology, Norwegian University of Life Sciences, Ås, Norway
A. Kohler: Faculty of Science and Technology, Norwegian University of Life Sciences, Ås, Norway
B. Mizaikoff: Institute of Analytical and Bioanalytical Chemistry, Ulm University, Ulm, Germany; Hahn-Schickard, Ulm, Germany
M. Finnilä: Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland
L. Rieppo: Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland
S. Saarakkala: Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland; Department of Diagnostic Radiology, Oulu University Hospital, Oulu, Finland

Journal volume & issue: Vol. 4, no. 2
p. 100250

Abstract

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Objective: To evaluate the feasibility of Fourier transform infrared attenuated total reflectance (FTIR-ATR) spectroscopy to detect cartilage degradation due to osteoarthritis and to validate the methodology with osteochondral human cartilage samples for future development towards clinical use. Design: Cylindrical (d = 4 mm) osteochondral samples (n = 349) were prepared from nine human cadavers and measured with FTIR-ATR spectroscopy. Afterwards, the samples were assessed with Osteoarthritis Research Society International (OARSI) osteoarthritis cartilage histopathology assessment system and divided into two groups: 1) healthy (OARSI 0–2) and 2) osteoarthritic (OARSI 2.5–6). The classification was done with partial least squares discriminant analysis model utilizing cross-model validation. Receiver operating characteristics curve analysis was performed and the area under curve (AUC) was calculated. Results: For all samples combined, classification accuracy was 73% with AUC of 0.79. Femoral samples had accuracy of 74% and AUC of 0.77, while tibial samples had accuracy of 66%, and AUC of 0.74. Patellar samples had accuracy of 84% and AUC of 0.91. Conclusions: The results indicate that FTIR-ATR spectroscopy can differentiate between healthy and osteoarthritic femoral, tibial and patellar human tissue. If combined with a fiber optic probe, FTIR-ATR spectroscopy could provide additional objective intraoperative information during arthroscopic surgeries, which could improve clinical outcomes.

Published in Osteoarthritis and Cartilage Open

ISSN: 2665-9131 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the musculoskeletal system
Website: https://www.journals.elsevier.com/osteoarthritis-and-cartilage-open/

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