Quartz Crystal Microbalance Genosensing of <i>Brettanomyces bruxellensis</i> Yeast in Wine Using a Rapid and Efficient <i>Drop and Collect</i> Protocol
Simone Poggesi,
Lan Zhou,
Giuliocesare Casari Bariani,
Rakesh Mittapalli,
Marisa Manzano,
Rodica Elena Ionescu
Affiliations
Simone Poggesi
Light, nanomaterials, nanotechnologies (L2n) Laboratory, CNRS ERL 7004, University of Technology of Troyes, 12 rue Marie Curie, CS 42060, CEDEX, 10004 Troyes, France
Lan Zhou
Light, nanomaterials, nanotechnologies (L2n) Laboratory, CNRS ERL 7004, University of Technology of Troyes, 12 rue Marie Curie, CS 42060, CEDEX, 10004 Troyes, France
Giuliocesare Casari Bariani
Light, nanomaterials, nanotechnologies (L2n) Laboratory, CNRS ERL 7004, University of Technology of Troyes, 12 rue Marie Curie, CS 42060, CEDEX, 10004 Troyes, France
Rakesh Mittapalli
Light, nanomaterials, nanotechnologies (L2n) Laboratory, CNRS ERL 7004, University of Technology of Troyes, 12 rue Marie Curie, CS 42060, CEDEX, 10004 Troyes, France
Marisa Manzano
Dipartimento di Scienze Agroalimentari, Ambientali e Animali (DI4A), Università degli Studi di Udine, Via Sondrio 2/A, 33100 Udine, Italy
Rodica Elena Ionescu
Light, nanomaterials, nanotechnologies (L2n) Laboratory, CNRS ERL 7004, University of Technology of Troyes, 12 rue Marie Curie, CS 42060, CEDEX, 10004 Troyes, France
A miniaturized quartz crystal microbalance (QCM) genosensor is proposed for sensitive and real-time detection of short ssDNA sequences (53 bp) or DNA extracted from Brettanomyces bruxellensis (Brett) yeast cells. The presence of Brett yeast causes a depreciation of the quality of aged fine wines, producing molecules of unpleasant odors and biogenic amines that are harmful to human health. More specifically, standard quartz crystal (S-QCM) and homemade 4 nm gold transmission electron microscopy (TEM)-grid patterned quartz (multi-TEM QCM) are herein proposed for biofunctionalization steps with different ssDNA sequences. By employing a rapid and efficient drop and collect protocol, the specific detection of 1 pg/µL ssDNA Brett of a short sequence and 100 ng/μL DNA of B. bruxellensis extracted from a wine sample (VR2008) is reported.
