Generation of a set of induced pluripotent stem cell lines from two Alzheimer disease patients carrying APOE4 (MLUi007-J; MLUi008-A) and healthy old donors carrying APOE3 (MLUi009-A; MLUi010-B) to study APOE in aging and disease

Matthias Jung; Carla Hartmann; Toni Ehrhardt; Lisa-Maria Peter; Chaudhry Luqman Abid; Bernadette Harwardt; Jana Hirschfeld; Claudia Claus; Undine Haferkamp; Ole Pless; Marina Nastainczyk-Wulf; Astrid Kehlen; Dietmar Schlote; Insa S. Schroder; Dan Rujescu

Stem Cell Research (Jun 2023)

Generation of a set of induced pluripotent stem cell lines from two Alzheimer disease patients carrying APOE4 (MLUi007-J; MLUi008-A) and healthy old donors carrying APOE3 (MLUi009-A; MLUi010-B) to study APOE in aging and disease

Matthias Jung,
Carla Hartmann,
Toni Ehrhardt,
Lisa-Maria Peter,
Chaudhry Luqman Abid,
Bernadette Harwardt,
Jana Hirschfeld,
Claudia Claus,
Undine Haferkamp,
Ole Pless,
Marina Nastainczyk-Wulf,
Astrid Kehlen,
Dietmar Schlote,
Insa S. Schroder,
Dan Rujescu

Affiliations

Matthias Jung: Institute for Physiological Chemistry, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06114 Halle (Saale), Germany; Corresponding author at: Institute for Physiological Chemistry, Medical Faculty of the Martin Luther University Halle-Wittenberg, Hollystrasse 1, 06114 Halle (Saale), Germany.
Carla Hartmann: Institute for Physiological Chemistry, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06114 Halle (Saale), Germany
Toni Ehrhardt: Institute for Physiological Chemistry, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06114 Halle (Saale), Germany
Lisa-Maria Peter: Institute for Physiological Chemistry, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06114 Halle (Saale), Germany
Chaudhry Luqman Abid: Institute for Physiological Chemistry, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06114 Halle (Saale), Germany
Bernadette Harwardt: Institute for Physiological Chemistry, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06114 Halle (Saale), Germany
Jana Hirschfeld: Institute for Physiological Chemistry, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06114 Halle (Saale), Germany
Claudia Claus: Insitute for Medical Microbiology and Virology, Medical Faculty, University of Leipzig, 04103 Leipzig, Germany
Undine Haferkamp: Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Discovery Research ScreeningPort, 22525 Hamburg, Germany
Ole Pless: Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Discovery Research ScreeningPort, 22525 Hamburg, Germany
Marina Nastainczyk-Wulf: Institute for Forensic Medicine, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06112 Halle (Saale), Germany
Astrid Kehlen: Department of Laboratory Medicine, Unit III, Molecular Diagnostic Section, Halle University Hospital, 06097 Halle (Saale), Germany
Dietmar Schlote: Human Genetics Department, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06112 Halle (Saale), Germany
Insa S. Schroder: Department of Biophysics, GSI Helmholtz Center for Heavy Ion Research, 64291 Darmstadt, Germany
Dan Rujescu: Department of Psychiatry and Psychotherapy, Division of General Psychiatry, Medical University of Vienna, 1090 Vienna, Austria

Journal volume & issue: Vol. 69
p. 103072

Abstract

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Late-onset Alzheimer disease (LOAD) is the most frequent neurodegenerative disease, and the APOE ε4 allele is the most prominent risk factor for LOAD. Four human induced pluripotent stem cell (iPSC) lines MLUi007-J, MLUi008-B, MLUi009-A, and MLUi010-B were generated from LOAD patients and healthy matched donors by reprogramming of B-lymphoblastoid cells (B-LCLs) with episomal plasmids. The application of B-LCLs holds a great promise to model LOAD and other diseases because they can easily be generated from primary peripheral blood mononuclear cells (PBMCs) by infection with the Epstein-Barr virus (EBV).

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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