GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS

Abstract

Aim. To study the state of gut microsymbiocenosis in children with reactive arthritis (RA), with the assessment of biofilm formation (BFF) of microsymbionts and the ability to change cytokine levels (their anticytokine activity) in vitro. Materials and methods. The investigation of gut microsymbiocenosis by means of bacteriological method was conducted in 34 children with RA and 25 relatively healthy 3 - 16 year- old children. Microorganisms were identified with the help of MALDI-TOF mass-spectrometry, anticytokine activity (АСА) of microsymbionts - according to Bukharin O.V. et al. (2011), biofilm formation - according to O’Toole G.A., Kolter R. (1998). Results. On the ground of species composition differences of gut microbiota discrimination model was created which allowed to separate the group of children with RA from healthy individuals. Microsymbiocenosis of patients with RAwas characterized by increasing number of opportunistic microorganisms (OM) (enterobacteria, clostridia, bacteroides, and Candida), BFF and АСА level. Conclusion. The obtained data greatly contribute to the deciphering of spondylo-arthritis and disclose the role of microbial factor under given pathology. Hypercolonisation of human gut with OM, having pronounced ability to BFF and regulating cytokine level, promotes strengthening of arthritogenic potential and serves as additional marker of arthritis development risk in children.

Keywords

• gut microsymbiocenosis
• anticytokine activity
• biofilm formation
• reactive arthritis
• children