Біологічні студії (Sep 2017)

Sulfidogenic activity of Desulfuromusa sp. SV30 under the influence of chromium, copper and iron compounds

  • S. V. Diakiv,
  • S. O. Hnatush,
  • V. V. Babenko,
  • O. M. Moroz

DOI
https://doi.org/10.30970/sbi.1102.530
Journal volume & issue
Vol. 11, no. 2
pp. 53 – 66

Abstract

Read online

Coal pit waste heap is an example of the multifactorial stress influence on organisms. The effect of heavy metals on the cell is the most toxic. Factor analysis of data for 29 variables, defined for gangues from coal pit waste heaps, was conducted. In the case of the analysis of red gangue four latent factors are revealed, it explains over 97 % total dispersion data. In the case of turfed by the mosses gangue as well as gangue without mosses analysis six latent factors were revealed, it explain about 87 and 86 % total dispersion data respectively. The ability of sulfur reducing bacteria Desulfuromusa sp. SV30, isolated from coal pits waste heaps of Chervonograd mining region, to utilize Cr (VI), Cu (II) and Fe (III) compounds as electron acceptors was studied. The influence of these compounds on growth and sulfidogenic activity of these bacteria was established. Desulfuromusa sp. СВ30 reduces 0.5–2 мМ of K2Cr2O7 and CuCl2, 1–10 мМ C6H5O7Fe as electron acceptors with varying intensity. 1 mМ of CuCl2 and C6H5O7Fe are completely reduced by bacteria. Desulfuromusa sp. SV30 incubation with heavy metals compounds (0.5 mM of K2Cr2O7, 1−2 mМ of CuCl or CuCl2, 1 mМ Fe2+ and 1−30 mМ Fe3+) causes bacteria metabolism intensification. Oxidized metals forms applying into the medium with sulfur at concentrations of 0.5 mМ (CuCl2) and 1 mМ leads to the higher biomass growth but sulfur reducing is strictly inhibited. At the presence of 0.5–1 мМ CuCl2 in medium 95.0−100.0 % of Cu2+ are bounded with HS- produced by the bacteria. After 1 mМ of FeCl2×4H2O applying into the medium the level of Fe2+ bounding with HS- equals 88.0 %. Indicated concentrations exceeding inhibited growth and sulfidogenic activity of Desulfuromusa sp. SV30. Highly effective application of Desulfuromusa sp. SV30 in technologies for purifying the environment from toxic sulfur- and metal-containing compounds is expected.

Keywords