SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand
Jae Min Choi,
Eunil Hahm,
Kyeonghui Park,
Daham Jeong,
Won-Yeop Rho,
Jaehi Kim,
Dae Hong Jeong,
Yoon-Sik Lee,
Sung Ho Jhang,
Hyun Jong Chung,
Eunae Cho,
Jae-Hyuk Yu,
Bong-Hyun Jun,
Seunho Jung
Affiliations
Jae Min Choi
Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Application (UBITA), Konkuk University, Seoul 05029, Korea
Eunil Hahm
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea
Kyeonghui Park
Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul 05029, Korea
Daham Jeong
Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul 05029, Korea
Won-Yeop Rho
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea
Jaehi Kim
Department of Chemistry Education, Seoul National University, Seoul 08826, Korea
Dae Hong Jeong
Department of Chemistry Education, Seoul National University, Seoul 08826, Korea
Yoon-Sik Lee
School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea
Sung Ho Jhang
Department of Physics, Konkuk University, Seoul 05029, Korea
Hyun Jong Chung
Department of Physics, Konkuk University, Seoul 05029, Korea
Eunae Cho
Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Application (UBITA), Konkuk University, Seoul 05029, Korea
Jae-Hyuk Yu
Departments of Bacteriology and Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA
Bong-Hyun Jun
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea
Seunho Jung
Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Application (UBITA), Konkuk University, Seoul 05029, Korea
Ethylenediamine-modified β-cyclodextrin (Et-β-CD) was immobilized on aggregated silver nanoparticle (NP)-embedded silica NPs (SiO2@Ag@Et-β-CD NPs) for the effective detection of flavonoids. Silica NPs were used as the template for embedding silver NPs to create hot spots and enhance surface-enhanced Raman scattering (SERS) signals. Et-β-CD was immobilized on Ag NPs to capture flavonoids via host-guest inclusion complex formation, as indicated by enhanced ultraviolet absorption spectra. The resulting SiO2@Ag@Et-β-CD NPs were used as the SERS substrate for detecting flavonoids, such as hesperetin, naringenin, quercetin, and luteolin. In particular, luteolin was detected more strongly in the linear range 10−7 to 10−3 M than various organic molecules, namely ethylene glycol, β-estradiol, isopropyl alcohol, naphthalene, and toluene. In addition, the SERS signal for luteolin captured by the SiO2@Ag@Et-β-CD NPs remained even after repeated washing. These results indicated that the SiO2@Ag@Et-β-CD NPs can be used as a rapid, sensitive, and selective sensor for flavonoids.
