Nature Communications (Jan 2021)
A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses
- Tiong Kit Tan,
- Pramila Rijal,
- Rolle Rahikainen,
- Anthony H. Keeble,
- Lisa Schimanski,
- Saira Hussain,
- Ruth Harvey,
- Jack W. P. Hayes,
- Jane C. Edwards,
- Rebecca K. McLean,
- Veronica Martini,
- Miriam Pedrera,
- Nazia Thakur,
- Carina Conceicao,
- Isabelle Dietrich,
- Holly Shelton,
- Anna Ludi,
- Ginette Wilsden,
- Clare Browning,
- Adrian K. Zagrajek,
- Dagmara Bialy,
- Sushant Bhat,
- Phoebe Stevenson-Leggett,
- Philippa Hollinghurst,
- Matthew Tully,
- Katy Moffat,
- Chris Chiu,
- Ryan Waters,
- Ashley Gray,
- Mehreen Azhar,
- Valerie Mioulet,
- Joseph Newman,
- Amin S. Asfor,
- Alison Burman,
- Sylvia Crossley,
- John A. Hammond,
- Elma Tchilian,
- Bryan Charleston,
- Dalan Bailey,
- Tobias J. Tuthill,
- Simon P. Graham,
- Helen M. E. Duyvesteyn,
- Tomas Malinauskas,
- Jiandong Huo,
- Julia A. Tree,
- Karen R. Buttigieg,
- Raymond J. Owens,
- Miles W. Carroll,
- Rodney S. Daniels,
- John W. McCauley,
- David I. Stuart,
- Kuan-Ying A. Huang,
- Mark Howarth,
- Alain R. Townsend
Affiliations
- Tiong Kit Tan
- MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
- Pramila Rijal
- MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
- Rolle Rahikainen
- Department of Biochemistry, University of Oxford
- Anthony H. Keeble
- Department of Biochemistry, University of Oxford
- Lisa Schimanski
- MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
- Saira Hussain
- Worldwide Influenza Centre, The Francis Crick Institute
- Ruth Harvey
- Worldwide Influenza Centre, The Francis Crick Institute
- Jack W. P. Hayes
- The Pirbright Institute
- Jane C. Edwards
- The Pirbright Institute
- Rebecca K. McLean
- The Pirbright Institute
- Veronica Martini
- The Pirbright Institute
- Miriam Pedrera
- The Pirbright Institute
- Nazia Thakur
- The Pirbright Institute
- Carina Conceicao
- The Pirbright Institute
- Isabelle Dietrich
- The Pirbright Institute
- Holly Shelton
- The Pirbright Institute
- Anna Ludi
- The Pirbright Institute
- Ginette Wilsden
- The Pirbright Institute
- Clare Browning
- The Pirbright Institute
- Adrian K. Zagrajek
- The Pirbright Institute
- Dagmara Bialy
- The Pirbright Institute
- Sushant Bhat
- The Pirbright Institute
- Phoebe Stevenson-Leggett
- The Pirbright Institute
- Philippa Hollinghurst
- The Pirbright Institute
- Matthew Tully
- The Pirbright Institute
- Katy Moffat
- The Pirbright Institute
- Chris Chiu
- The Pirbright Institute
- Ryan Waters
- The Pirbright Institute
- Ashley Gray
- The Pirbright Institute
- Mehreen Azhar
- The Pirbright Institute
- Valerie Mioulet
- The Pirbright Institute
- Joseph Newman
- The Pirbright Institute
- Amin S. Asfor
- The Pirbright Institute
- Alison Burman
- The Pirbright Institute
- Sylvia Crossley
- The Pirbright Institute
- John A. Hammond
- The Pirbright Institute
- Elma Tchilian
- The Pirbright Institute
- Bryan Charleston
- The Pirbright Institute
- Dalan Bailey
- The Pirbright Institute
- Tobias J. Tuthill
- The Pirbright Institute
- Simon P. Graham
- The Pirbright Institute
- Helen M. E. Duyvesteyn
- Division of Structural Biology, Nuffield Department of Medicine, University of Oxford, The Wellcome Centre for Human Genetics
- Tomas Malinauskas
- Division of Structural Biology, Nuffield Department of Medicine, University of Oxford, The Wellcome Centre for Human Genetics
- Jiandong Huo
- Division of Structural Biology, Nuffield Department of Medicine, University of Oxford, The Wellcome Centre for Human Genetics
- Julia A. Tree
- National Infection Service, Public Health England
- Karen R. Buttigieg
- National Infection Service, Public Health England
- Raymond J. Owens
- Division of Structural Biology, Nuffield Department of Medicine, University of Oxford, The Wellcome Centre for Human Genetics
- Miles W. Carroll
- National Infection Service, Public Health England
- Rodney S. Daniels
- Worldwide Influenza Centre, The Francis Crick Institute
- John W. McCauley
- Worldwide Influenza Centre, The Francis Crick Institute
- David I. Stuart
- Centre for Translational Immunology, Chinese Academy of Medical Sciences Oxford Institute, University of Oxford
- Kuan-Ying A. Huang
- Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University
- Mark Howarth
- Department of Biochemistry, University of Oxford
- Alain R. Townsend
- MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
- DOI
- https://doi.org/10.1038/s41467-020-20654-7
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 16
Abstract
Vaccines for SARS-COV-2 are needed in the ongoing pandemic. Here the authors characterize a vaccine candidate that presents the receptor-binding domain (RBD) of SARS-CoV-2 spike protein on a synthetic VLP platform using SpyTag/SpyCatcher technology and show immunogenicity of a prime-boost regimen in mice and pigs.
