Evaluating Predictors of Quality in Liver NK Cells Among Deceased Donors

Yuki Imaoka; Masahiro Ohira; Toshihiro Nakayama; Miho Akabane; Tetsuya Tajima; Shinichiro Yokota; Sheri M. Krams; Olivia M. Martinez; Carlos O. Esquivel; Kazunari Sasaki; Hideki Ohdan

doi:10.1177/09636897241283289

Cell Transplantation (Oct 2024)

Evaluating Predictors of Quality in Liver NK Cells Among Deceased Donors

Yuki Imaoka,
Masahiro Ohira,
Toshihiro Nakayama,
Miho Akabane,
Tetsuya Tajima,
Shinichiro Yokota,
Sheri M. Krams,
Olivia M. Martinez,
Carlos O. Esquivel,
Kazunari Sasaki,
Hideki Ohdan

Affiliations

Yuki Imaoka: Division of Abdominal Transplant, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA
Masahiro Ohira: Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan
Toshihiro Nakayama: Division of Abdominal Transplant, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA
Miho Akabane: Division of Abdominal Transplant, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA
Tetsuya Tajima: Division of Abdominal Transplant, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA
Shinichiro Yokota: Division of Abdominal Transplant, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA
Sheri M. Krams: Division of Abdominal Transplant, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA
Olivia M. Martinez: Division of Abdominal Transplant, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA
Carlos O. Esquivel: Division of Abdominal Transplant, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA
Kazunari Sasaki: Division of Abdominal Transplant, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA
Hideki Ohdan: Department of Gastroenterological and Transplant Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan

DOI: https://doi.org/10.1177/09636897241283289
Journal volume & issue: Vol. 33

Abstract

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This study investigated the substantial variability in the quality of liver natural killer (NK) cells from deceased donors (DDs). This study assessed liver nonparenchymal cells from 51 DDs for activation receptors and cytotoxicity against K562 (leukemia) and HepG2 (hepatoma) cell lines. The results indicated variability in TNF-related apoptosis-inducing ligand (TRAIL) and NK stimulatory receptor NK group 2 member D (NKG2D) expression in liver NK cells from DDs, which correlated with cytotoxicity against tumor cell lines. In addition, the white blood cell (WBC) count, aspartate aminotransferase (AST) level, body mass index (BMI), and platelet count were significantly associated with enhanced TRAIL and NKG2D expression. A predictive score integrating AST/platelet ratio index, BMI, and WBC count was developed to effectively identify DDs with high antitumor activity in liver NK cells. This score is expected to predict DDs with high-quality liver NK cells, which can be used for the purpose of immunotherapies.

Published in Cell Transplantation

ISSN: 0963-6897 (Print); 1555-3892 (Online)
Publisher: SAGE Publishing
Country of publisher: United States
LCC subjects: Medicine
Website: http://journals.sagepub.com/home/cll

About the journal