Chiral Monolithic Silica-Based HPLC Columns for Enantiomeric Separation and Determination: Functionalization of Chiral Selector and Recognition of Selector-Selectand Interaction
Mufarreh Asmari,
Xiaoyu Wang,
Natalia Casado,
Marjan Piponski,
Sergiy Kovalenko,
Liliya Logoyda,
Rasha Sayed Hanafi,
Sami El Deeb
Affiliations
Mufarreh Asmari
College of Pharmacy, King Khalid University, Abha 62529, Saudi Arabia
Xiaoyu Wang
Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA
Natalia Casado
Departamento de Tecnología Química y Ambiental, E.S.C.E.T, Universidad Rey Juan Carlos, C/Tulipán s/n, Móstoles, 28933 Madrid, Spain
Marjan Piponski
Replek Farm Ltd., st. Kozle 188, 1000 Skopje, North Macedonia
Sergiy Kovalenko
Department of Organic and Bioorganic Chemistry, Zaporizhzhia State Medical University, Maiakovskyi avenue 26, 69035 Zaporizhzhia, Ukraine
Liliya Logoyda
Department of Pharmaceutical Chemistry, I. Horbachevsky Ternopil National Medical University, Maidan Voli 1, 46001 Ternopil, Ukraine
Rasha Sayed Hanafi
Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, Egypt
Sami El Deeb
Institute of Medicinal and Pharmaceutical Chemistry, Technische Universität Braunschweig, 38106 Braunschweig, Germany
This review draws attention to the use of chiral monolithic silica HPLC columns for the enantiomeric separation and determination of chiral compounds. Properties and advantages of monolithic silica HPLC columns are also highlighted in comparison to conventional particle-packed, fused-core, and sub-2-µm HPLC columns. Nano-LC capillary monolithic silica columns as well as polymeric-based and hybrid-based monolithic columns are also demonstrated to show good enantioresolution abilities. Methods for introducing the chiral selector into the monolithic silica column in the form of mobile phase additive, by encapsulation and surface coating, or by covalent functionalization are described. The application of molecular modeling methods to elucidate the selector–selectand interaction is discussed. An application for enantiomeric impurity determination is also considered.
