Glucose: a possible intermediate in the oxidation of the side chain of cholesterol in resting and stimulated rats

M.R. MALINOW; N. BAKER; PHYLLIS McLAUGHLIN; ANNE PERLEY

Journal of Lipid Research (Nov 1971)

Glucose: a possible intermediate in the oxidation of the side chain of cholesterol in resting and stimulated rats

M.R. MALINOW,
N. BAKER,
PHYLLIS McLAUGHLIN,
ANNE PERLEY

Affiliations

M.R. MALINOW: Oregon Regional Primate Research Center, Beaverton, Oregon 97005;; University of Oregon Medical School, Portland, Oregon 97001; Radioisotope Research, Veterans Administration Hospital, Los Angeles, California 90073;; Department of Biological Chemistry, University of California School of Medicine, Los Angeles, California 90024
N. BAKER: Oregon Regional Primate Research Center, Beaverton, Oregon 97005;; University of Oregon Medical School, Portland, Oregon 97001; Radioisotope Research, Veterans Administration Hospital, Los Angeles, California 90073;; Department of Biological Chemistry, University of California School of Medicine, Los Angeles, California 90024
PHYLLIS McLAUGHLIN: Oregon Regional Primate Research Center, Beaverton, Oregon 97005;; University of Oregon Medical School, Portland, Oregon 97001; Radioisotope Research, Veterans Administration Hospital, Los Angeles, California 90073;; Department of Biological Chemistry, University of California School of Medicine, Los Angeles, California 90024
ANNE PERLEY: Oregon Regional Primate Research Center, Beaverton, Oregon 97005;; University of Oregon Medical School, Portland, Oregon 97001; Radioisotope Research, Veterans Administration Hospital, Los Angeles, California 90073;; Department of Biological Chemistry, University of California School of Medicine, Los Angeles, California 90024

Journal volume & issue: Vol. 12, no. 6
pp. 747 – 759

Abstract

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The effect of repeated muscular contraction on the rate of oxidation of the side chain of cholesterol was studied in anesthetized rats. The animals received an intravenous pulse-label injection of either cholesterol-26-14C, incorporated into rat plasma lipoproteins, or bicarbonate-14C. In half the animals of each group, the hind legs were repeatedly stimulated by electrical impulses. A multicompartmental analysis was attempted, based on the disappearance curve of plasma free cholesterol-14C and on the excretion rate of expired 14CO2, as well as on previously reported rates of bile acid and adrenal steroid secretion. The rate of expired 14CO2 originating from cholesterol-26-14C was much less than that predicted by the digital computer analysis; cholesterol degradation could not be evaluated since the data were incompatible with a model that assumes direct oxidation of the side chain to CO2. A revised model was postulated in which an important fraction of the side chain of cholesterol would be converted to CO2 only after previous conversion to glucose. Direct measurement of plasma glucose-14C after the injection of cholesterol-26-14C supported this hypothesis.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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