Journal of Biological Researches (Feb 2012)

Analisis pestisida karbaril dengan metode KLT-Densitometri dalam matriks kedelai dan validasi metodenya

  • Sugijanto Erma Sugijanto,
  • Prihatin Erma Prihatin,
  • Noor Erma Sugijanto N

DOI
https://doi.org/10.23869/bphjbr.15.2.201010
Journal volume & issue
Vol. 15, no. 2
pp. 165 – 169

Abstract

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A simple and rapid densitometric method has been developed for determination of low levels of carbaryl in soy bean matrix samples.Sample carbaryl in the soybean matrix was extracted with acetonitril, chloroform and NaCl 0.2 % solution, and the resulting chloroformlayer was separated, added sodium sulphate anhydrous to get free of water than evaporated. After dissolution in methanol, the solutionswere spotted on silica gel GF254 plates which were then eluted with toluene: ethyl acetate = 3:1 (v/v). Quantitative evaluation wasperformed by measuring the absorbance reflectance of the analyte spots at λ 280 nm. This densitometric method is selective, precise,accurate and can be used for the determination carbaryl in soy bean matrix.Key words: carbaryl, TLC-densitometry, validation methode, soy bean

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