OsMYB84, a transcriptional regulator of OsCOPT2 and OsHMA5, modulates copper uptake and transport and yield production in rice
Jingli Ding,
Chenchen Ji,
Lu Yu,
Chuang Wang,
Guangda Ding,
Sheliang Wang,
Lei Shi,
Fangsen Xu,
Hongmei Cai
Affiliations
Jingli Ding
Research Center of Microelement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Chenchen Ji
Research Center of Microelement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Lu Yu
Research Center of Microelement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Chuang Wang
Research Center of Microelement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Guangda Ding
Research Center of Microelement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Sheliang Wang
Research Center of Microelement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Lei Shi
Research Center of Microelement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, Hubei, China; National Key Laboratory of Crop Genetics and Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Fangsen Xu
Research Center of Microelement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, Hubei, China; National Key Laboratory of Crop Genetics and Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Hongmei Cai
Research Center of Microelement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Corresponding author.
Transcription factors regulating crop uptake and translocation of the micronutrient Cu have not been identified. We isolated a novel R2R3-MYB transcription factor, OsMYB84, and showed that it was a positive regulator involved in uptake and transport of Cu via activation of OsCOPT2 and OsHMA expression. OsMYB84 was highly expressed in roots and anthers and induced by Cu. Overexpression of OsMYB84 promoted uptake and root-to-shoot translocation of Cu in rice, facilitated Cu distribution into grain and increased grain yield. In contrast, mutation of OsMYB84 reduced Cu concentration in xylem sap. OsMYB84 bound to the promoter region of OsCOPT2 and OsHMA5 and upregulated their expression. OsCOPT2 mutants showed reduced uptake of Cu and OsHMA5 overexpression lines showed increased root-to-shoot translocation of Cu.
