Nature Communications (Dec 2017)

Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

  • Florian Schueder,
  • Juanita Lara-Gutiérrez,
  • Brian J. Beliveau,
  • Sinem K. Saka,
  • Hiroshi M. Sasaki,
  • Johannes B. Woehrstein,
  • Maximilian T. Strauss,
  • Heinrich Grabmayr,
  • Peng Yin,
  • Ralf Jungmann

DOI
https://doi.org/10.1038/s41467-017-02028-8
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 9

Abstract

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Existing methods for nanoscale visualization of biological targets in thick samples require complex hardware. Here, the authors combine the standard spinning disk confocal (SDC) microscopy with DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) to image proteins, DNA and RNA deep in cells.