Preparation Method of Gene Chip for Rapid Qualitative Detection of Coronavirus inHuman Respiratory Samples and Its Effect Evaluation

Abstract

Objective To explore the development method of coronavirus gene chip, and to establish a method for rapid qualitative detection of nucleic acids of 7 coronaviruses and 2 influenza viruses in respiratory infection samples such as human throat swabs, sputum, and alveolar lavage fluid. Methods According to the gene sequences of 10 kinds of respiratory infection viruses published on the Internet, the corresponding primers and probe sequences were designed.After screening and verification by agarose gel electrophoresis, the probes were spotted on the aldehyde base modified substrate, completed the detection chip fabrication. Results The PCR amplification product was diluted by 10-fold serial dilution method and then detected by gene chip.The results showed that the lowest detection concentration that could form hybridization spots was 103copies/mL. Conclusion The developed coronavirus gene chip has good specificity and sensitivity, and can be used for simultaneous high-throughput and rapid detection of SARS-CoV-2 and other respiratory viruses.

Keywords

• coronavirus
• influenza virus
• gene chip