Emerging Infectious Diseases (Dec 2009)

Diagnostic Assay for Rickettsia japonica

  • Nozomu Hanaoka,
  • Minenosuke Matsutani,
  • Hiroki Kawabata,
  • Seigo Yamamoto,
  • Hiromi Fujita,
  • Akiko Sakata,
  • Yoshinao Azuma,
  • Motohiko Ogawa,
  • Ai Takano,
  • Haruo Watanabe,
  • Toshio Kishimoto,
  • Mutsunori Shirai,
  • Ichiro Kurane,
  • Ichiro Takajo

DOI
https://doi.org/10.3201/eid1512.090252
Journal volume & issue
Vol. 15, no. 12
pp. 1994 – 1997

Abstract

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We developed a specific and rapid detection system for Rickettsia japonica and R. heilongjiangensis, the causative agents of spotted fever, using a TaqMan minor groove binder probe for a particular open reading frame (ORF) identified by the R. japonica genome project. The target ORF was present only in R. japonica–related strains.

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