PLoS ONE (Jan 2022)

Distribution characteristics of the sabA, hofC, homA, homB and frpB-4 genes of Helicobacter pylori in different regions of China.

  • Mengyang Fang,
  • Zhijing Xue,
  • Lihua He,
  • Yuanhai You,
  • Yanan Gong,
  • Dongjie Fan,
  • Lu Sun,
  • Kangle Zhai,
  • Yaming Yang,
  • Jianzhong Zhang

DOI
https://doi.org/10.1371/journal.pone.0268373
Journal volume & issue
Vol. 17, no. 5
p. e0268373

Abstract

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BackgroundHelicobacter pylori (H. pylori) encodes numerous outer membrane proteins (OMPs), with considerable geographic heterogeneity and related to different clinical outcomes. This study aimed to investigate the distribution characteristics of five important OMP genes (sabA, hofC, homA, homB and frpB-4) in different regions of China.Materials and methodA total of 266 strains were isolated from 348 stomach biopsy specimens in Shandong, Guangxi, Heilongjiang, Hunan, and Qinghai provinces. The presence of sabA, hofC, homA, homB and frpB-4 gene was detected by polymerase chain reaction (PCR) from H. pylori genomic DNA.ResultsAmong the strains in five regions, the prevalence of frpB-4 was 100% and that of hofC was 97.7%. The prevalence of homB in the isolates from Qinghai (45.5%) was significantly lower than that in Shandong (75.3%), Guangxi (76.9%) and Hunan (69.6%) (PConclusionThe prevalence of homA, homB, and sabA genes and the sabA "on" or "off" status have significant geographical differences among five provinces in China. The presence of homA and sabA genes may be protective factors of gastritis.