Nature Communications (Sep 2019)

Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways

  • Aziz Taghbalout,
  • Menghan Du,
  • Nathaniel Jillette,
  • Wojciech Rosikiewicz,
  • Abhijit Rath,
  • Christopher D. Heinen,
  • Sheng Li,
  • Albert W. Cheng

DOI
https://doi.org/10.1038/s41467-019-12339-7
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 12

Abstract

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DNA methylation plays an important role in regulating a wide variety of cellular processes and is implicated in a range of diseases. Here the authors present Casilio-ME to assemble protein complexes to demethylate target loci.