The regulation of hsacirc_004413 promotes proliferation and drug resistance of gastric cancer cells by acting as a competing endogenous RNA for miR-145-5p
Fusheng Zhou,
Weiqun Ding,
Qiqi Mao,
Xiaoyun Jiang,
Jiajie Chen,
Xianguang Zhao,
Weijia Xu,
Jiaxin Huang,
Liang Zhong,
Xu Sun
Affiliations
Fusheng Zhou
Department of Gastroenterology, Huashan Hospital North, Fudan University, Shanghai, China
Weiqun Ding
Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, China
Qiqi Mao
Department of Gastroenterology, Huashan Hospital North, Fudan University, Shanghai, China
Xiaoyun Jiang
Department of Gastroenterology, Huashan Hospital North, Fudan University, Shanghai, China
Jiajie Chen
Department of Gastroenterology, Huashan Hospital North, Fudan University, Shanghai, China
Xianguang Zhao
Department of Gastroenterology, Huashan Hospital North, Fudan University, Shanghai, China
Weijia Xu
Department of Gastroenterology, Huashan Hospital North, Fudan University, Shanghai, China
Jiaxin Huang
Department of Gastroenterology, Huashan Hospital North, Fudan University, Shanghai, China
Liang Zhong
Department of Gastroenterology, Huashan Hospital North, Fudan University, Shanghai, China
Xu Sun
Department of Gastroenterology, Huashan Hospital North, Fudan University, Shanghai, China
Background Whether circRAN, which acts as a microRNA sponge, plays a role in 5-fluorouracil (5-Fu) resistant gastric cancer has not been reported. In this study, a 5-Fu resistant cell line with an IC50 of 16.59 µM was constructed. Methods Using comparative analysis of circRNA in the transcriptomics of resistant and sensitive strains, 31 differentially expressed circRNAs were detected, and the microRNA interacting with them was predicted. Results Hsacirc_004413 was selected for verification in drug resistant and sensitive cells. By interfering with hsacirc_004413 using antisense RNA, the sensitivity of drug resistant cells to 5-Fu was significantly promoted, and the apoptosis and necrosis of the cells were significantly increased. In sensitive cells, inhibition by inhibitors enhanced the resistance of cells to 5-Fu. We hypothesize that hsacirc_004413 makes gastric cancer cells resistant to 5-Fu mainly through adsorption of miR-145-5p.
