Minimal lactazole scaffold for in vitro thiopeptide bioengineering

Alexander A. Vinogradov; Morito Shimomura; Yuki Goto; Taro Ozaki; Shumpei Asamizu; Yoshinori Sugai; Hiroaki Suga; Hiroyasu Onaka

doi:10.1038/s41467-020-16145-4

Nature Communications (May 2020)

Minimal lactazole scaffold for in vitro thiopeptide bioengineering

Alexander A. Vinogradov,
Morito Shimomura,
Yuki Goto,
Taro Ozaki,
Shumpei Asamizu,
Yoshinori Sugai,
Hiroaki Suga,
Hiroyasu Onaka

Affiliations

Alexander A. Vinogradov: Department of Chemistry, Graduate School of Science, The University of Tokyo
Morito Shimomura: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Yuki Goto: Department of Chemistry, Graduate School of Science, The University of Tokyo
Taro Ozaki: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Shumpei Asamizu: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Yoshinori Sugai: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Hiroaki Suga: Department of Chemistry, Graduate School of Science, The University of Tokyo
Hiroyasu Onaka: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

DOI: https://doi.org/10.1038/s41467-020-16145-4
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

Read online

Lactazole A is a thiopeptide from Streptomyces lactacystinaeus, encoded by a compact 9.8 kb biosynthetic gene cluster. Here, the authors show a platform for in vitro biosynthesis of lactazole A via a combination of a flexible in vitro translation system with recombinantly produced lactazole biosynthetic enzymes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal