PSEUDOMONAS FLUORESCENTES ASSOCIADAS À CULTURA DE COUVE: INFLUÊNCIA DA ADUBAÇÃO

Abstract

The microbiota soil has an important role as an indicator of the sustainability of agroecosystems, reflecting the environmental changes, particularly the antrophic actions. To evaluate the influence of different fertilizers in populations of Pseudomonas spp, in the common kale was conducted a field experiment with kale at the Agrobiology Embrapa National Center, in Seropédica, RJ, on a Argisol. The experimental design was random blocks in factorial 3 x 4, with treatments (home biosolid, cattle manure and urea fertilization), four dose levels (0, 100, 200 and 400 kg de N.ha-1) and four replicates. The amount of fertilizer applied was given according to the dosage of nitrogen.ha-1 desired. From the rhizosphere, at 15 and 30 days after transplanting the seedlings to the field, we selected strains showing fluorescence under UV light with a wavelength of 366 nm. For grouping the isolates were considered the main morphological characteristics. The majority being identified as Pseudomonas putida (54%) and P. fluorescens (14%), by API 20NE System (bioMérieux, Analytab Products). The results obtained from the reactions of the API 20NE test kit showed a wide variation in the utilization of carbon compounds and enzymatic inter-and intraspecific. Some groups of isolates colonized preferentially the cabbage rhizosphere of plants fertilized with biosolids and different doses of fertilizers used. There were a smaller number of groups present in treatments with urea.