Analysis and Comparison of Rapid Methods for the Determination of Ochratoxin a Levels in Organs and Body Fluids Obtained from Exposed Mice
Zsuzsanna Szőke,
Bianka Babarczi,
Miklós Mézes,
István Lakatos,
Miklós Poór,
Eszter Fliszár-Nyúl,
Miklós Oldal,
Árpád Czéh,
Kornélia Bodó,
György Nagyéri,
Szilamér Ferenczi
Affiliations
Zsuzsanna Szőke
Institute of Genetics and Biotechnology, Department of Animal Biotechnology, Hungarian University of Agriculture and Life Sciences, Agribiotechnology and Precision Breeding for Food Security National Laboratory, Szent-Györgyi Albert Street 4, H-2100 Gödöllő, Hungary
Bianka Babarczi
Institute of Genetics and Biotechnology, Department of Animal Biotechnology, Hungarian University of Agriculture and Life Sciences, Agribiotechnology and Precision Breeding for Food Security National Laboratory, Szent-Györgyi Albert Street 4, H-2100 Gödöllő, Hungary
Miklós Mézes
Institute of Physiology and Nutrition, Department of Feed Safety, Hungarian University of Agriculture and Life Sciences, Páter K. Street 1, H-2100 Gödöllő, Hungary
István Lakatos
Institute of Genetics and Biotechnology, Department of Animal Biotechnology, Hungarian University of Agriculture and Life Sciences, Agribiotechnology and Precision Breeding for Food Security National Laboratory, Szent-Györgyi Albert Street 4, H-2100 Gödöllő, Hungary
Miklós Poór
Department of Pharmacology, Faculty of Pharmacy, University of Pécs, Szigeti Street 12, H-7624 Pécs, Hungary
Eszter Fliszár-Nyúl
Department of Pharmacology, Faculty of Pharmacy, University of Pécs, Szigeti Street 12, H-7624 Pécs, Hungary
Institute of Genetics and Biotechnology, Department of Animal Biotechnology, Hungarian University of Agriculture and Life Sciences, Agribiotechnology and Precision Breeding for Food Security National Laboratory, Szent-Györgyi Albert Street 4, H-2100 Gödöllő, Hungary
Szilamér Ferenczi
Institute of Experimental Medicine, Laboratory of Molecular Neuroendocrinology, Szigony Street 43, H-1083 Budapest, Hungary
Mycotoxins are bioaccumulative contaminants impacting animals and humans. The simultaneous detection of frequent active exposures and accumulated mycotoxin level (s) in exposed organisms would be the most ideal to enable appropriate actions. However, few methods are available for the purpose, and there is a demand for dedicated, sensitive, reliable, and practical assays. To demonstrate the issue, mice were exposed to a relevant agent Ochratoxin A (OTA), and accumulated OTA was measured by fine-tuned commercial assays. Quantitative high-performance liquid chromatography with fluorescence detection, enzyme-linked immunosorbent assay, and flow cytometry assays have been developed/modified using reagents available as commercial products when appropriate. Assays were performed on excised samples, and results were compared. Accumulated OTA could be detected and quantified; positive correlations (between applied doses of exposure and accumulated OTA levels and the results from assays) were found. Dedicated assays could be developed, which provided comparable results. The presence and accumulation of OTA following even a short exposure could be quantitatively detected. The assays performed similarly, but HPLC had the greatest sensitivity. Blood contained higher levels of OTA than liver and kidney. We demonstrate that specific but flexible and practical assays should be used for specific/local purposes, to measure the exposure itself and accumulation in blood or organs.