NASC-seq monitors RNA synthesis in single cells

Gert-Jan Hendriks; Lisa A. Jung; Anton J. M. Larsson; Michael Lidschreiber; Oscar Andersson Forsman; Katja Lidschreiber; Patrick Cramer; Rickard Sandberg

doi:10.1038/s41467-019-11028-9

Nature Communications (Jul 2019)

NASC-seq monitors RNA synthesis in single cells

Gert-Jan Hendriks,
Lisa A. Jung,
Anton J. M. Larsson,
Michael Lidschreiber,
Oscar Andersson Forsman,
Katja Lidschreiber,
Patrick Cramer,
Rickard Sandberg

Affiliations

Gert-Jan Hendriks: Department of Cell and Molecular Biology, Karolinska Instiutet, Biomedicum
Lisa A. Jung: Department of Biosciences and Nutrition, Karolinska Institutet, NEO
Anton J. M. Larsson: Department of Cell and Molecular Biology, Karolinska Instiutet, Biomedicum
Michael Lidschreiber: Department of Biosciences and Nutrition, Karolinska Institutet, NEO
Oscar Andersson Forsman: Department of Cell and Molecular Biology, Karolinska Instiutet, Biomedicum
Katja Lidschreiber: Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry
Patrick Cramer: Department of Biosciences and Nutrition, Karolinska Institutet, NEO
Rickard Sandberg: Department of Cell and Molecular Biology, Karolinska Instiutet, Biomedicum

DOI: https://doi.org/10.1038/s41467-019-11028-9
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 9

Abstract

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Sequencing of newly synthesised RNA can reveal the transcriptional dynamics in a population of cells. Here the authors develop NASC-seq to bring this sensitivity and temporal resolution to single-cell analysis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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