Communications Biology (Oct 2022)

Three-dimensional characterization of developing and adult ocular vasculature in mice using in toto clearing

  • Marie Darche,
  • Anna Verschueren,
  • Morgane Belle,
  • Leyna Boucherit,
  • Stéphane Fouquet,
  • José Alain Sahel,
  • Alain Chédotal,
  • Ilaria Cascone,
  • Michel Paques

DOI
https://doi.org/10.1038/s42003-022-04104-2
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 10

Abstract

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An organic-solvent based clearing technique is used to visualize the vasculature in 3D using light sheet microscopy in retinal and hyaloid vessel development, and applied to branch retinal vein occlusion (BRVO) models.