Cancer Management and Research (Dec 2020)
Circ-PRKDC Facilitates the Progression of Colorectal Cancer Through miR-198/DDR1 Regulatory Axis
Abstract
Guixiang Wang,1 Yajun Li,1 Hufei Zhu,1 Guoqiang Huo,1 Jingying Bai,1 Zhiyong Gao2 1Department of Colorectal Surgery, Yan’an People’s Hospital, Yan’an, Shaanxi, People’s Republic of China; 2Department of General Surgery, Yanchuan County People’s Hospital, Yan’an, Shaanxi, People’s Republic of ChinaCorrespondence: Zhiyong Gao Tel +86-911-2888754Email [email protected]: Circular RNAs (circRNAs) play a crucial role in a variety of cancers, including colorectal cancer (CRC). This study aimed to explore the role of hsa_circ_0136666 (circ-PRKDC) in CRC and its potential mechanism.Methods: The levels of circ-PRKDC, miR-198 and discoidin domain receptor 1 (DDR1) were measured using quantitative real-time polymerase chain reaction or Western blot. Cell viability was detected using cell counting kit-8 (CCK-8) assay. Cell apoptosis and cycle were evaluated via flow cytometry. Cell migration and invasion were examined using transwell assay. CyclinD1 protein level was determined via Western blot. The interaction among circ-PRKDC, miR-198 and DDR1 was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation assay. Xenograft assay was performed to analyze tumor growth in vivo.Results: Circ-PRKDC and DDR1 levels were increased, and miR-198 level was decreased in CRC tissues and cells. Circ-PRKDC depletion inhibited proliferation, migration and invasion, and expedited apoptosis and cell cycle arrest in SW480 and HCT116 cells. Silence of circ-PRKDC impeded CRC progression by sponging miR-198. Overexpression of miR-198 hindered CRC development via targeting DDR1. Moreover, circ-PRKDC silencing suppressed tumor growth in vivo.Conclusion: Knockdown of circ-PRKDC inhibited CRC progression via modulating miR-198/DDR1 pathway.Keywords: colorectal cancer, circ-PRKDC, miR-198, DDR1, cell cycle
