Italian Journal of Animal Science (Dec 2024)

A comparative study on semen quality and cryopreservation ability in Italian native chicken breeds

  • Michele Di Iorio,
  • Stefano Paolo Marelli,
  • Emanuele Antenucci,
  • Manuela Madeddu,
  • Luisa Zaniboni,
  • Silvia Belcredito,
  • Giusy Rusco,
  • Achille Schiavone,
  • Dominga Soglia,
  • Mauro Penasa,
  • Cesare Castellini,
  • Arianna Buccioni,
  • Margherita Marzoni,
  • Lucia Maiuro,
  • Nicolaia Iaffaldano,
  • Silvia Cerolini

DOI
https://doi.org/10.1080/1828051X.2024.2424518
Journal volume & issue
Vol. 23, no. 1
pp. 1704 – 1718

Abstract

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This study provides a comparative assessment of fresh semen quality and sensitivity to cryopreservation in several Italian chicken breeds. The research involved 145 roosters from 13 breeds. The results showed a wide variability in all the parameters considered among the different breeds, especially in the quantitative variables of fresh semen, such as volume and concentration. For the qualitative characteristics (sperm membrane integrity and motility parameters), the variability across breeds was more pronounced for frozen than fresh semen. Interestingly, apart from total motility in fresh semen, breed had a significant effect on all semen quality parameters in both fresh and thawed ejaculates. Considering the overall qualitative characteristics, the Robusta maculata, Siciliana, and Mericanel della Brianza breeds produced ejaculates with better semen quality compared to other Italian breeds. By evaluating the main parameters of semen quality, our results underline the potential of these traits to influence the reproductive success and genetic conservation. The Bionda piemontese, Bianca di Saluzzo, Livorno bianca, Pepoi, and Siciliana breeds showed better resilience to cryopreservation, suggesting the need for breed-specific protocols to optimise semen quality after thawing. Importantly, the research highlights the central role of semen quality for both immediate fertilisation success and long-term conservation efforts. Future studies integrating OMICS technologies could elucidate molecular markers influencing breed-specific differences, helping to refine cryopreservation techniques and improve conservation strategies for indigenous Italian chicken breeds. This work contributes valuable insights to global efforts aimed at safeguarding poultry genetic diversity and sustainability.

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