Molecular basis for PrimPol recruitment to replication forks by RPA

Thomas A. Guilliam; Nigel C. Brissett; Aaron Ehlinger; Benjamin A. Keen; Peter Kolesar; Elaine M. Taylor; Laura J. Bailey; Howard D. Lindsay; Walter J. Chazin; Aidan J. Doherty

doi:10.1038/ncomms15222

Nature Communications (May 2017)

Molecular basis for PrimPol recruitment to replication forks by RPA

Thomas A. Guilliam,
Nigel C. Brissett,
Aaron Ehlinger,
Benjamin A. Keen,
Peter Kolesar,
Elaine M. Taylor,
Laura J. Bailey,
Howard D. Lindsay,
Walter J. Chazin,
Aidan J. Doherty

Affiliations

Thomas A. Guilliam: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Nigel C. Brissett: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Aaron Ehlinger: Departments of Biochemistry and Chemistry and Center for Structural Biology, Vanderbilt University School of Medicine
Benjamin A. Keen: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Peter Kolesar: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Elaine M. Taylor: Lancaster Medical School, Faculty of Health and Medicine, Lancaster University
Laura J. Bailey: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Howard D. Lindsay: Lancaster Medical School, Faculty of Health and Medicine, Lancaster University
Walter J. Chazin: Departments of Biochemistry and Chemistry and Center for Structural Biology, Vanderbilt University School of Medicine
Aidan J. Doherty: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex

DOI: https://doi.org/10.1038/ncomms15222
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 14

Abstract

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PrimPol is a multifunctional replicative enzyme that can bypass DNA damage, as well as reprime replication restart. Here, the authors have elucidated how PrimPol is recruited to stalled replication forks via specific interactions with RPA, which stimulates its primase activity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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