TALEN outperforms Cas9 in editing heterochromatin target sites

Surbhi Jain; Saurabh Shukla; Che Yang; Meng Zhang; Zia Fatma; Manasi Lingamaneni; Shireen Abesteh; Stephan Thomas Lane; Xiong Xiong; Yuchuan Wang; Charles M. Schroeder; Paul R. Selvin; Huimin Zhao

doi:10.1038/s41467-020-20672-5

Nature Communications (Jan 2021)

TALEN outperforms Cas9 in editing heterochromatin target sites

Surbhi Jain,
Saurabh Shukla,
Che Yang,
Meng Zhang,
Zia Fatma,
Manasi Lingamaneni,
Shireen Abesteh,
Stephan Thomas Lane,
Xiong Xiong,
Yuchuan Wang,
Charles M. Schroeder,
Paul R. Selvin,
Huimin Zhao

Affiliations

Surbhi Jain: Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana−Champaign
Saurabh Shukla: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana−Champaign
Che Yang: Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana−Champaign
Meng Zhang: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana−Champaign
Zia Fatma: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana−Champaign
Manasi Lingamaneni: Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana−Champaign
Shireen Abesteh: Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana−Champaign
Stephan Thomas Lane: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana−Champaign
Xiong Xiong: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana−Champaign
Yuchuan Wang: Computational Biology Department, School of Computer Science, Carnegie Mellon University
Charles M. Schroeder: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana−Champaign
Paul R. Selvin: Center for the Physics of Living Cells, University of Illinois at Urbana−Champaign
Huimin Zhao: Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana−Champaign

DOI: https://doi.org/10.1038/s41467-020-20672-5
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 10

Abstract

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While Cas9 outperforms TALENs in euchromatin, it is less efficient in heterochromatic regions. Here the authors, using single-molecule imaging, show that Cas9 uses a less efficient search strategy compared to TALENs in these regions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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