Human Genomics (Jun 2006)

Genetic factors leading to chronic Epstein-Barr virus infection and nasopharyngeal carcinoma in South East China: Study design, methods and feasibility

  • Guo Xiu,
  • Scott Kevin,
  • Liu Yan,
  • Dean Michael,
  • David Victor,
  • Nelson George W,
  • Johnson Randall C,
  • Dilks Holli H,
  • Lautenberger James,
  • Kessing Bailey,
  • Martenson Janice,
  • Guan Li,
  • Sun Shan,
  • Deng Hong,
  • Zheng Yuming,
  • de The Guy,
  • Liao Jian,
  • Zeng Yi,
  • O'Brien Stephen J,
  • Winkler Cheryl A

DOI
https://doi.org/10.1186/1479-7364-2-6-365
Journal volume & issue
Vol. 2, no. 6
pp. 365 – 375

Abstract

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Abstract Nasopharyngeal carcinoma (NPC) is a complex disease caused by a combination of Epstein-Barr virus chronic infection, the environment and host genes in a multi-step process of carcinogenesis. The identity of genetic factors involved in the development of chronic Epstein-Barr virus infection and NPC remains elusive, however. Here, we describe a two-phase, population-based, case-control study of Han Chinese from Guangxi province, where the NPC incidence rate rises to a high of 25-50 per 100,000 individuals. Phase I, powered to detect single gene associations, enrolled 984 subjects to determine feasibility, to develop infrastructure and logistics and to determine error rates in sample handling. A microsatellite screen of Phase I study participants, genotyped for 319 alleles from 34 microsatellites spanning an 18-megabase region of chromosome 4 (4p15.1-q12), previously implicated by a linkage analysis of familial NPC, found 14 alleles marginally associated with developing NPC or chronic immunoglobulin A production (p = 0.001-0.03). These associations lost significance after applying a correction for multiple tests. Although the present results await confirmation, the Phase II study population has tripled patient enrolment and has included environmental covariates, offering the potential to validate this and other genomic regions that influence the onset of NPC.

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